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翻訳と辞書　辞書検索 [ 開発暫定版 ] スポンサード リンク 4Pi STED microscopy ： ウィキペディア英語版 4Pi STED microscopy The 4Pi-STED-microscope is the result of combining the two unrelated concepts of STED- and 4Pi-microscopy. Here, the fluorescent sample is placed in the common focus of two opposing lenses, but excitation and detection are performed through a single lens (4Pi mode A). The green excitation pulse is immediately followed by a red STED-pulse, which enters the focal region through both lenses inducing stimulated emission of the excited fluorescent molecules to the ground state. To permit fluorescence emission from the center but suppress it from neighbouring regions it is useful to phaseshift the STED beam to have a minimum at the center. ==External links== * (Technical details ) 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「4Pi STED microscopy」の詳細全文を読む スポンサード リンク 翻訳と辞書 : 翻訳のためのインターネットリソース Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.